About us

The company Protean s.r.o. was based in 2008 as a consortium of investors and specialists in biotechnology and molecular biology in order to develop a private subject aimed to contribute to the development of biotechnological industry in the South Bohemia. We are dedicated to support research and development of advanced diagnostic tools both for human and veterinary medicine, based on the implementation of last scientific achievements. Besides that we intensively collaborate on several research projects together with state institutions and universities.

Our employees

Doc. RNDr. Ladislav Burýšek, PhD
Research & Development Director

Professional carrier:

Laboratory of Immunology, Research Institute of Endocrinology, Prague, Czech Republic	1989-1991
Institute of Biochemistry and Medical chemistry, University of Bari, Bari, Italy	1993
Institute of Physiology, ASCR, Prague, Czech Republic	1991-1997
Johns Hopkins University, Oncology Center, Baltimore, Maryland, USA, postdoctoral stay	1997-2000
Department of Pharmacology of Natural Products and Clinical Pharmacology, University Hospital of Ulm, Ulm, Germany, postdoctoral stay, habilitation	2000-2005
GEN-TREND s.r.o., Research & Development Director	2005-present

Grant projects solved:

Grant Agency of the Czech Republic: Regulation of expression and function of pyrogenic cytokines in somatic cells.	1995-1997
National Institute of Health, USA: Cloning and characterization of novel Kaposi’s sarcoma herpes virus-encoded interferon regulatory factors.	1997-2000
University of Ulm, Germany: Cloning and characterization of human homologue of viral interferon regulatory factor 2 (vIRF-2).	2001-2003
Deutsches Forschunggesellschaft (DFG), Germany: Characterization of virus-induced transcription factors and regulation of gene expression of the host cell.	2002-2004
Ministry of Industry and Trade of the Czech Republic, Project Impulse: Development and manufacture of new generation of molecular biological diagnostic kits for parallel "macroarray" detection of human pathogens.	2004-2006
German-Israeli Project Cooperation (DIP), The German Ministry for Education and Research (BMBF), Germany: The role of intracellular IL-1 alpha in health and disease: mechanisms of action and therapeutic applications.	2005

Primary papers in peer reviewed journals:

Svecova, L., Vrzal, R., Burysek, L., Anzenbacherova, E., Cerveny, L., Grim, J., Trejtnar, F., Kunes, J., Pour, M., Staud, F., Anzenbacher, P., Dvorak, Z., Pavek, P. (2007) Azole antimycotics differentially affect rifampicin-induced Pregnane X Receptor (PXR)-mediated CYP3A4 gene expression. Drug Metab Dispos. 2007 Nov 12; [Epub ahead of print]

Laumonnier, Y., Syrovets, T., Burysek, L., Simmet, T. (2006) Identification of the annexin A2 heterotetramer as a receptor for the plasmin-induced signaling in human peripheral monocytes. Blood.

Buryskova, M., Pospisek, M., Grothey, A., Simmet, T., Burysek, L. (2004) Intracellular interleukin-1alpha functionally interacts with histone acetyltransferase complexes. J Biol Chem. 279, 4017-4026.

Colognato, R., Slupsky, J.R., Jendrach, M., Burysek, L., Syrovets, T., Simmet, T. (2003) Differential expression and regulation of protease-activated receptors in human peripheral monocytes and monocyte-derived antigen-presenting cells. Blood 102, 2645-2652.

Smola, S.F., Rettenberger, G., Simmet, T., Burysek, L. (2003) Comparison of sample collection methods for the PCR detection of oral anaerobic pathogens. Lett Appl Microbiol 36, 101-105.

Burysek, L., Syrovets, T., Simmet, T. (2002) The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signaling pathways. J Biol Chem 277, 33509-33517

Burysek, L., and Pitha, P. M. (2001). Latently Expressed Human Herpesvirus 8-encoded Interferon Regulatory Factor-2 (vIRF-2) Inhibits dsRNA-activated Protein Kinase (PKR). J Virol 75, 2345-2352.

Burysek, L., Yeow, W. S., Lubyova, B., Kellum, M., Schafer, S. L., Huang, Y. Q., and Pitha, P. M. (1999). Functional analysis of human herpesvirus 8-encoded viral interferon regulatory factor 1 and its association with cellular interferon regulatory factors and p300. J Virol 73, 7334-7342.

Burysek, L., Yeow, W. S., and Pitha, P. M. (1999). Unique properties of a second human herpesvirus 8-encoded interferon regulatory factor (vIRF-2). J Hum Virol 2, 19-32. GENBANK/AF045550

Pitha, P. M., Au, W. C., Lowther, W., Juang, Y. T., Schafer, S. L., Burysek, L., Hiscott, J., and Moore, P. A. (1998). Role of the interferon regulatory factors (IRFs) in virus-mediated signaling and regulation of cell growth. Biochimie 80, 651-8.

Funda, D. P., Houstek, J., Holub, M., Kazdova, L., Michalsky, M., Burysek, L., Cervinkova, M., and Sima, P. (1998). Differences in thermoregulation between immunocompetent and immunodeficient hairless mice exposed to mild cold. Folia Microbiol 43, 487-489.

Burysek, L., and Houstek, J. (1997). beta-Adrenergic stimulation of interleukin-1alpha and interleukin-6 expression in mouse brown adipocytes. FEBS Lett 411, 83-86.

Burysek, L., and Houstek, J. (1996). Multifactorial induction of gene expression and nuclear localization of mouse interleukin 1 alpha. Cytokine 8, 460-467.

Burysek, L. (1996). The role of pyrogenic cytokines in brown fat. In Department of Bioenergetics, Institute of Physiology, ASCR (Prague: Charles University), pp. 96.

Burysek, L., Tvrdik, P., and Houstek, J. (1993). Expression of interleukin-1 alpha and interleukin-1 receptor type I genes in murine brown adipose tissue. FEBS Lett 334, 229-232.